A power calculation based on earlier studies suggested that 6 animals in each group would be sufficient to detect a statistically significant difference in bacterial count, which was the primary outcome in this study. Group I - rats with uninfected wounds submitted to topical treatment with 0. Group II - infected wounds treated with saline. Group III - uninfected wounds treated with honey.
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- The Antibacterial Activity of Honey!
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- The Antibacterial Activity of Honey.
Group IV - infected wounds treated with honey. Using a 1. The pocket was closed by means of nylon sutures and this procedure resulted in a local abscess after 24 h. The rats were returned to individual cages and they were examined daily. After 24 h, the wounds were opened, the gauze removed for quantitative bacterial cultures and treatment started. Postoperative pain was controled with meperidine Roche Farma, Brazil. It was then covered with plain gauze. In groups I and II the same volume of sterile saline was used.
This treatment was applied once a day for 7 days. Quantization of viable bacteria was performed by culturing ten-fold dilutions of each sample. A 1cm x 2cm area of skin, including the wound was excised aseptically.